Fig. 4.
Intracellular accumulation of IFN-γ, IL-5, TNF-α, and GM-CSF in CD56+ and CD56− NK cells from cultures of Lin− cells to determine the sequence with which the ability to produce type 1 and type 2 cytokines is acquired during differentiation.

Intracellular accumulation of IFN-γ, IL-5, TNF-α, and GM-CSF in CD56+ and CD56 NK cells from cultures of Lin cells to determine the sequence with which the ability to produce type 1 and type 2 cytokines is acquired during differentiation.

Cells from primary cultures of Lin cells with Sl/Sl4hSCF220 feeder cells and IL-2 (left panels) or IL-15 (right panels) were stimulated as in Figure 3. Intracellular cytokines and surface phenotype were analyzed simultaneously (3-color immunofluorescence) on gated CD161+cells within purified CD3 cells using FITC-, PE-, or biotin-labeled mAbs to the indicated molecules and streptavidin-R670. Quadrants were set to distinguish CD56+ and CD56 cells. Percent positive cells is indicated in each quadrant. Experiment representative of 4 performed with similar results.

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