HA is selectively transported to the uropod in polarized T lymphoblasts.

Human T lymphoblasts or Jurkat cells were infected with influenza virus for 4 hours at 37°C. Unpermeabilized T lymphoblasts were fixed and subjected to double labeling with mouse anti-HA mAb and rabbit anti–ICAM-3 antibodies. After permeabilization with 0.2% Triton X-100, T-cell blasts were analyzed with filipin to stain the cellular cholesterol, or double-labeled with anti-Golgi 58K and anti–ICAM-3 antibodies. Spherical blasts and Jurkat cells (bottom panels) were labeled with anti-HA antibodies in the absence of permeabilization step. To show cell morphology bright field images are depicted. Bar is 10 μm.