American Society of Hematology

Fig. 8.

$Fig. 8. Ploidy MKs release more platelets than diploid MKs. / (A) MKs from PB culture were sorted on the basis of the cell size at day 8. The R1 obtained population (∼10%) was recultured in serum-free medium in the presence of TPO (100 ng/mL). (B) Morphology of the megakaryocytic-sorted fraction after Wright-Giemsa staining (Bar = 30 μm). (C) ▪: percentage of proplatelets forming MKs on PB-R1 fraction and day 12 CB-derived MKs. ■: platelets produced by the same populations were enumerated at days 12 and 14, respectively. (See also “Materials and methods” and “Results.”)$

Ploidy MKs release more platelets than diploid MKs.

(A) MKs from PB culture were sorted on the basis of the cell size at day 8. The R1 obtained population (∼10%) was recultured in serum-free medium in the presence of TPO (100 ng/mL). (B) Morphology of the megakaryocytic-sorted fraction after Wright-Giemsa staining (Bar = 30 μm). (C) ▪: percentage of proplatelets forming MKs on PB-R1 fraction and day 12 CB-derived MKs. ■: platelets produced by the same populations were enumerated at days 12 and 14, respectively. (See also “Materials and methods” and “Results.”)

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