Effect of anti-SAA antibody on platelet adhesion to SAA.

(A) SAA- and fibronectin-coated microtiter plate wells were preincubated with monoclonal anti-SAA antibody mc29 (500 μg/mL) for 1 hour, after which washed platelets were added and adhesion assay was performed, as described. One representative experiment of 2 performed is shown. (B) SAA (0.1 μg and 1 μg) and C5a (1 μg) were subjected to Western blot analysis by using mc29 antibody. C5a is an unrelated protein that is not recognized by this antibody.