Progressive clearance of Bcl-2/IgH-positive cells after rituximab administration.

PCR analysis was performed at the indicated time points on patients' BM (▪) and PB (●) mononuclear cells. As negative control, a sample containing all PCR reagents without DNA was amplified in parallel with DNA extracted from PB mononuclear cells obtained from a pool of healthy donors. In each experiment, DNA extracted at diagnosis and DNA from the Bcl-2/IgH-positive DoHH2 cell line were used as positive controls.