HGF-induced proliferation of MM cells.
(A) HGF mediates increased survival in XG-1. Cells were grown in the absence of IL-6 and serum, and HGF was added at a concentration of 200 ng/mL. Viability was measured by FACS analysis on days 0, 3, 5, and 7, using propidium iodide incorporation. On day 0, relative viability was set at 100%. (B) HGF is a potent growth factor for XG-1. Culture conditions were as in (A), HGF was added in the concentration shown, and the number of viable cells was quantified by using propidium iodide incorporation and FACS analysis at days 0, 3, 5, and 7. (C) HGF induces proliferation in XG-1. Cells were cultured as in (A), and HGF was added in the concentrations shown. 3H thymidine incorporation was measured on days 3 and 4. Error bars represent the SD of a triplicate measurement.
