Effects of monensin and brefeldin A on recovery of CCR5.

Internalization was initiated in CHO.CCR5 cells with MIP-1α, as described, and cells were washed and incubated in medium in the presence or absence of monensin and brefeldin A for 120 minutes. Cells were then subjected to FACS analysis. 1, control; 2, 120 minutes; 3, monensin, 120 minutes; 4, brefeldin A, 120 minutes; 5, brefeldin A plus monensin, 120 minutes. Data represent mean ± SEM of at least 3 independent experiments.