Marked difference in the ability of PB and BM CD4⁺ and CD8⁺ T cells to induce lethal GVH disease.

Graded numbers of sorted CD4⁺, CD8⁺, or CD4⁺/CD8⁺ (CD4⁺ and CD8⁺ together as a pool) T cells from C57BL/6 donors were added to a constant number (1.5 × 10⁶) of C57BL/6 TCD BM cells and injected intravenously into lethally irradiated (8 Gy) BALB/c hosts. Survival of hosts over a 100-day observation period is shown in groups of 10 mice. Data are combined from 2 replicate experiments. (A) Graded numbers of sorted PB and BM CD4⁺ T cells. (B) Graded numbers of PB and BM CD8⁺ T cells. (C) Graded numbers of PB and BM CD4⁺/CD8⁺ T cells.