Two-color flow cytometric analysis of TCRαβ⁺ T cells from PB and BM of C57BL/6 mice.

(A) Staining of TCRαβ versus CD4 or CD8. (B) The analysis of the gated TCRαβ⁺CD4⁺ or TCRαβ⁺CD8⁺ cells from panel A for NK1.1 versus CD4 or CD8. (C) One-color analysis of the gated NK1.1⁻ CD4⁺TCRαβ⁺ from PB (open profiles) or BM (shaded profiles) for CD44, CD62L, and CD45RB. (D) One-color analysis of the gated NK1.1⁻CD8⁺ TCRαβ⁺ T cells. Percentages of gated cells are shown above the boxes. One representative of 4 is shown.