Fig. 6.
![Fig. 6. Pulse chase analysis of β3 integrin biosynthesis. / Wild-type, His280Pro, or Arg216Gln/Leu292Serβ3 cDNA was cotransfected into 293 cells with either (A) wild-type αIIb cDNA or (B) wild-type αv cDNA. Cells were labeled one day after transfection with 0.4 mCi/mL (14.8 MBq) [35S]methionine for 30 minutes and chased with media containing 50 μg/mL of nonradioactive methionine for various periods of time as indicated. Immunoprecipitation was performed using either (A) TP80 or (B) LM142. Precipitates were separated by 6% SDS-PAGE under reducing conditions. Results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/3/10.1182_blood.v99.3.931/5/h80322098006.jpeg?Expires=1722564457&Signature=e7wqK~60m~AXopv7hExSSnexI6VR-48HAaMK8N2Nd7DNIHVeFxxwafegoTOIx8uFRQJTwDCgKK9oJR2nNf62vOiE19z5hGofFuU7SFw~PpI~ahFDCAfqSMsKqYY5dDnx3eNtnwvyNGDLV56d6ZQz4u70TDTYAhaAf4JtefnCohHaPOeK64G78vQ2HZXCF0UC4nd5Nlw3JF8lsXIkGw26eOdP~cFA-7iNWOlIHAaHRcZ6qGH2RcnmnbjVTj3N4S5m6sXvi9E21b3UezMBJbRRAiytW2iDNd1kqZoVZetpfCkJxqaR3WqQQLwrYrBxWawJFvWP~3G5-Qfg8mTK-iyLjg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse chase analysis of β3 integrin biosynthesis.
Wild-type, His280Pro, or Arg216Gln/Leu292Serβ3 cDNA was cotransfected into 293 cells with either (A) wild-type αIIb cDNA or (B) wild-type αv cDNA. Cells were labeled one day after transfection with 0.4 mCi/mL (14.8 MBq) [35S]methionine for 30 minutes and chased with media containing 50 μg/mL of nonradioactive methionine for various periods of time as indicated. Immunoprecipitation was performed using either (A) TP80 or (B) LM142. Precipitates were separated by 6% SDS-PAGE under reducing conditions. Results are representative of 3 separate experiments.
