Fig. 8.
Kinetics of 5-LO product formation.
Freshly isolated PMNLs (5 × 107 in 10 mL PGC buffer) were stimulated either with SA (10 μM) 3 minutes prior to addition of 40 μM AA (●), or with ionophore A2387 (2.5 μM) together with AA (○). After the indicated times at 37°C, aliquots of these incubations (1 mL) were added to the same volume of ice-cold methanol to stop the 5-LO reaction, and 5-LO products were determined. Results are given as mean + SE (n = 3).
