American Society of Hematology

Fig. 3.

$Fig. 3. SA induces rapid translocation of 5-LO to the nucleus. / Freshly isolated PMNLs (3 × 107 in 1 mL PGC buffer) were stimulated with varying concentrations of SA or 2.5 μM ionophore A23187 at 37°C for the indicated times. After cell fractionation by detergent lysis (0.1% NP-40), 5-LO was determined in nuclear fractions by immunoblotting. Results are representative of at least 3 separate experiments.$

SA induces rapid translocation of 5-LO to the nucleus.

Freshly isolated PMNLs (3 × 10⁷ in 1 mL PGC buffer) were stimulated with varying concentrations of SA or 2.5 μM ionophore A23187 at 37°C for the indicated times. After cell fractionation by detergent lysis (0.1% NP-40), 5-LO was determined in nuclear fractions by immunoblotting. Results are representative of at least 3 separate experiments.

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