The −41/−17 region is essential for the murine

RAG-2 promoter in B-cell lines. (A) Diagram of promoter/luciferase construct. Mouse RAG-2 gene spanning −1.1 kb to +147 bp or its serial deletion (open box for −1.1 kb to −1 and shaded box for +1 to +147) was linked to 5′ of luciferase reporter gene (closed box). +1 indicates major transcription initiation site. (B) Promoter activity in B-cell lines with serially deletedRAG-2 promoter region. A quantity of 10 μg luciferase constructs linked to the serially deleted mouse RAG-2promoter region (indicated on left) was transfected into 18.8.1 cells or BAL17 cells. pSRα-LacZ was included as an internal control. Twenty-four hours later, luciferase and β-galactosidase assays were performed on cell extracts. The activity of the luciferase construct without promoter in each cell is set to 1. Error bars indicate deviation of 3 experiments.