Fig. 7.
![Fig. 7. Expression of differentiation markers MPO and NG in bone marrow granulocyte colonies. / (A) Expression of MPO (black staining) is shown in the cytoplasm of granulocytes from agar colonies stimulated by EG-R (Ai) WT, (Aii) Y704→F, (Aiii) Y729→F, (Aiv) Y744→F, (Av) Y764→F, and (Avi) Ynull (× 1000). (B) Expression of NG mRNA (207 base pair [bp]) in granulocytes was detected by RT-PCR. As a control, bone marrow cells isolated from G-CSF–deficient mice (−/−) were also analyzed. (C) Negative control with no RNA. Expression of β-actin (605 bp) was shown as a control. This figure is representative of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/3/10.1182_blood.v99.3.879/5/h80322091007.jpeg?Expires=1722541561&Signature=TAKrVgIgfZc0KHCnpOlaLC-EcIOCF38X4jc5F-Mm~i7S50H9NOVRAD-Nkw47P1We0z9f1FmQwuzLq2ApPmKodHddGMZrTJIOTm38F2rXKVtqDWyNSgTWnvC26RtuxT8e0b7pk0THadsVTWaZ25njCFf41SRhYFrZuOuvqurI0cKdqu9PNRqv-TlllkCdmerbOSM1NfpIW1PKPzUirrHf50cZhgSwNatZ2CfT9WmvbaME4lHTy~SaUCFmfr9Nbx5OVBml1BA7ACcNesZnZ3nrfkzBssllKlDRQSoB3zALakNIvRVuI~t3q~BXFBi2~KcYvOjwaEIgtqoqwN0X9O8CgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of differentiation markers MPO and NG in bone marrow granulocyte colonies.
(A) Expression of MPO (black staining) is shown in the cytoplasm of granulocytes from agar colonies stimulated by EG-R (Ai) WT, (Aii) Y704→F, (Aiii) Y729→F, (Aiv) Y744→F, (Av) Y764→F, and (Avi) Ynull (× 1000). (B) Expression of NG mRNA (207 base pair [bp]) in granulocytes was detected by RT-PCR. As a control, bone marrow cells isolated from G-CSF–deficient mice (−/−) were also analyzed. (C) Negative control with no RNA. Expression of β-actin (605 bp) was shown as a control. This figure is representative of 2 independent experiments.
