SDF-1 stimulates IL-10 production by peritoneal B cells.

(A) Peritoneal cells (black columns) or spleen cells (gray columns) were cultured either alone or in the presence of various SDF-1 concentrations. IL-10 concentration was determined in the supernatant. Results (mean ± SEM) are from 3 experiments. (B-D) Peritoneal cells were incubated with or without SDF-1. They were then labeled with CD19, CD5, Mac-1 mAb, and with an anti–IL-10 mAb. (B) B1a cells; (C) B1b cells; (D) B2 cells. Results (mean ± SEM from 6 experiments) are the proportion of cells containing IL-10. No labeling of IL-10 was detected if an FITC-coupled control mAb was used instead of an anti–IL-10 mAb or if the cell permeabilization step was omitted (data not shown). *P < .05 and **P < .005 compared with cells cultured in the absence of SDF-1.