Enhanced vascular leakage in the inflamed skin of TSP-2–deficient mice.

No major differences were found in the extent of plasma extravasation in untreated skin between wild-type mice (A) and TSP-2–deficient mice (B), as evaluated after intravenous injection of Evans blue. In contrast, topical application of mustard oil to the ear skin resulted in a more pronounced vascular leakage of Evans blue in TSP-2–deficient mice (D), as compared with wild-type mice (C). To quantify the plasma extravasation, Evans blue was extracted from the skin. No major differences were found in the amount of Evans blue extravasation in untreated skin of TSP-2–deficient (▪) and wild-type (■) mice (E), whereas leakage was increased more than 2.4-fold in the inflamed skin of TSP-2–deficient mice, as compared with wild-type mice. Data are expressed as mean ± SD. *P < .05.