CD150 mutation/deletion constructs used in this study.

(A) CD150. The 3 SAP consensus sequences surrounding Tyr281, Tyr307, and Tyr327 are indicated. (B) CD150 point mutants, containing tyrosine-phenylalanine substitutions in the SAP-binding motifs. (C) CD150 point mutants, containing threonine-alanine substitutions at position −2 to the central tyrosine in the SAP binding motif. (D) Chimeric CD8-CD150/CD3ε constructs encoding the ectodomain of CD8 and the cytoplasmic and membrane domains of CD150.