Induction of IL-6 expression by LANA.

(A) IL-6 and TNF-α levels in culture supernatants of 293 cells stably transfected with EGFP-LANA or EGFP blank vectors. Experiments were performed in duplicate. TNF-α levels were undetectable in 293 cells transfected with EGFP-LANA or the EGFP control vector. IL-6 levels were undetectable in 293 cells transfected with the EGFP control vector. (B) IL-6 levels in culture supernatants of R1T cells expressing EGFP-LANA in the tetracycline-regulated system. Various concentrations of doxycycline were used to induce EGFP-LANA expression; 1 μg/mL doxycycline represents a suppressive concentration. Experiments were performed in duplicate.