Fig. 2.
![Fig. 2. Internal deletion mapping. / Schematic representation of JK alleles from the Jknull donor [JK*A(Δ4,5)] and from a control Jk-positive donor [JK*A or JK*B allele]. Exons are indicated by rectangles. Filled and open symbols correspond to coding and noncoding sequences, respectively. Primers used for PCR-1 are indicated. S, SacI. (right) Gel analysis of PCR-1 products. The junction sequence found in the Jknull donor and the 5′ and 3′ breakpoints of the deletion are indicated (bottom). Sequence alignment of the wild type (wt) and deleted DNA from the Jknull donor around the deletion breakpoint showing the small direct repeats (shadowed).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/3/10.1182_blood.v99.3.1079/5/h80322087002.jpeg?Expires=1722729868&Signature=QU8Ke0Tnmj4vj3OehTbzb2~sPXLp~KRmw-mBnaOAi0G7pRTf040KeGYcQezcTW-1fFfjZ5fI18-9Oa5U4N1gJUDEpESUz8tnwBMIFRZC1syrJ3-mXr-phNtUYWq03CEUmGaZMIVKBrSJZkkcXkqbeXokbHzvE8yFsvjT~JNIucZwJCE4PLaBmNwhUcXPJu4c3tKm4yb-g0c-MKMpyOys4rUiBGKHNLIelhAp6yAv3qA8sDD9kVVG9ZSCihLZC7fN70XxHPFl~bN8y3f64LGActIrd2DUySAi3vRkIf~v0Hw36KqwNbFvYILzbFS9~52HTXcrPFEfkfly5pRZmOCB9A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Internal deletion mapping.
Schematic representation of JK alleles from the Jknull donor [JK*A(Δ4,5)] and from a control Jk-positive donor [JK*A or JK*B allele]. Exons are indicated by rectangles. Filled and open symbols correspond to coding and noncoding sequences, respectively. Primers used for PCR-1 are indicated. S, SacI. (right) Gel analysis of PCR-1 products. The junction sequence found in the Jknull donor and the 5′ and 3′ breakpoints of the deletion are indicated (bottom). Sequence alignment of the wild type (wt) and deleted DNA from the Jknull donor around the deletion breakpoint showing the small direct repeats (shadowed).
