RBC membrane proteins, genomic DNA, and Jk transcript from the Jk_null and control Jk(a+b+) donors.

(A) Immunoblot analysis. RBC membrane proteins (40 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with antibodies against the N-terminal or C-terminal of the Jk/hUT-B1 protein.9 (B) Southern blot analysis: Genomic DNA (15 μg/lane) was digested with SacI (10 U/μg), resolved by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with a [³²P]-labeled cDNA probe (exons 4 to 11).2 Size of DNA markers (kb) and exons in each fragment are given in the left and right margins, respectively. (C) Partial sequence analysis. Jk_null transcript sequence from Alf-Express DNA sequencer (Amersham Pharmacia, Uppsala, Sweden). E*, intronic sequence found in the Jk(Δ4,5) transcript from the propositus but not in the Jk(a+b+) control. Genomic sequences were deposited to the European Molecular Biology Laboratory (EMBL) database under the accession numbers AJ316564 and AJ316565.