Fig. 1.
![Fig. 1. PNU156804 disrupts cell growth of activated, but not of unactivated, human T cells in a dose-dependent manner. / Proliferation of quiescent PHA-activated human T cells (5.0 × 104 cells/well) in the presence of 1 nM IL-2 was examined after treatment with increasing concentrations of PNU156804 (●) or inactive control PNU159744 (♦) for 16 hours at 37°C. Conversely, Jurkat cells were treated in an identical fashion with PNU156804 (▴). All cells were then pulsed with [3H]-thymidine (0.5 μCi [0.0185 MBq]/200 μL) for 4 hours, incorporated into a radiolabeled probe, and plotted on the abscissa expressed as total cpm (n = 6).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/2/10.1182_blood.v99.2.680/6/h80222002001.jpeg?Expires=1722776459&Signature=0uLA8ajO3eb7L5xSXARnG9uKWo7t1vwv3MP6J1jw6ogI3jaheKrPVc5M5mXM9XtMgqil~heqwBRIjLJLujQ5ZbQjZKTFzDmvJ~ejNI-iyKWqTY14lAXLFXLqg~6mAPNvj0fCl1d4ocFCBBZNn~NxgQVwnt1yUHbavqlZfSYPAMmREoipw5P2WsETIsuzGv-4heI1FKnWX46MxRZuGbMEn07s8wfahss--HcLhrvBfZzXkWQ6d6o15Zgrr-ibnqizX7UR1MoIyHJt9lYRQ6wul8eVzJsrIa6xWCjzFOq8Ui39G35DK7CaoKLBViDzQ4c2WZ90mDHxmNlhMqqx5y8Iiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PNU156804 disrupts cell growth of activated, but not of unactivated, human T cells in a dose-dependent manner.
Proliferation of quiescent PHA-activated human T cells (5.0 × 104 cells/well) in the presence of 1 nM IL-2 was examined after treatment with increasing concentrations of PNU156804 (●) or inactive control PNU159744 (♦) for 16 hours at 37°C. Conversely, Jurkat cells were treated in an identical fashion with PNU156804 (▴). All cells were then pulsed with [3H]-thymidine (0.5 μCi [0.0185 MBq]/200 μL) for 4 hours, incorporated into a radiolabeled probe, and plotted on the abscissa expressed as total cpm (n = 6).
