Ultrastructure of cells derived from monocytes cultured in the presence of IFN-β and IL-3.

Transmission electron microscopy of monocytes after culture for 6 days with either GM-CSF and IL-4 or IL-3 and IFN-β. IL-3–IFN-β DCs and GM-CSF–IL-4 DCs showed the typical appearance of dendritic cells, including a lobulated nucleus, long cytoplasmic processes, and tubulovesicular system. In the presence of IL-3 and IFN-β DCs appeared as smaller cells filled with mitochondria.