Fig. 1.
Characterization of tBOECs.
tBOECs were characterized before they were administered to mice. At this time, they had expanded approximately 106-fold since gene transfer and approximately 1012-fold overall since the establishment of initial BOEC culture from blood. Panels illustrate morphology and uptake of acetylated LDL, negativity for monocyte marker CD14, positivity for the transgene product FVIII, and positivity for multiple endothelial cell markers. The illustrated control peak for unlabeled cells was identical to the control peak for isotype control antibody in all cases.
