Fig. 2.
Fig. 2. Optimizing the amount of anti–TGF-β antibody to decrease p15 protein levels. / UCB CD34+ progenitors were incubated for 18 hours in serum-free media alone or in the presence of increasing concentrations of anti–TGF-β antibody (ng/mL). Cell lysates were prepared and subjected to 15% SDS-PAGE, followed by immunoblotting with a polyclonal antibody to p15Ink4b.

Optimizing the amount of anti–TGF-β antibody to decrease p15 protein levels.

UCB CD34+ progenitors were incubated for 18 hours in serum-free media alone or in the presence of increasing concentrations of anti–TGF-β antibody (ng/mL). Cell lysates were prepared and subjected to 15% SDS-PAGE, followed by immunoblotting with a polyclonal antibody to p15Ink4b.

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