Effect of size on pulmonary targeting and effects of GOX conjugates (anti-PECAM/GOX and anti-TM/GOX) in intact mice.

Blood level (black bars) and pulmonary uptake (gray bars) of small (250-nm, left part) versus large (600-nm, right part) anti-TM/¹²⁵I-GOX or IgG/¹²⁵I-GOX conjugates (2 to 3 μg per mouse) 1 hour after intravenous injection (panel A; accumulation data of anti-PECAM/¹²⁵I-GOX in murine lung are not shown). Pathological changes in the lungs harvested 3 hours after IV injection of 50 μg per mouse of small (C,F,I) or large (D,G,J) anti-TM/GOX conjugate versus saline injection (B,E,H). Panels B, C, and D show lung gross pathology; note severe hemorrhagic lung injury in panel C. Panels E, F, and G show hematoxylin/eosin staining of lung tissue sections; note edema and vascular injury in panel F. Panels H, I, and J show immunostaining for the products of lipid peroxidation; note positive reaction (blue color) in panel I. Original magnifications, panels E-J, × 40. Tissue edema determined as lung wet-to-dry ratio (K) and ALIS (L) in the lungs harvested 3 hours after IV injection of 50 μg per mouse of small (200-nm) or large (700-nm) anti-PECAM/GOX conjugate versus saline injection (control).