Phenotype of NLCs.

Cells were harvested after 14 days of culture and analyzed by flow cytometry. The upper left panel depicts a scatter plot of the side-angle (y-axis) and forward-angle (x-axis) light scatter of leukemic lymphocytes or NLCs, as indicated by the arrows. The dots within the broken line represent the light scatter of individual NLCs. The other panels depict the logarithmic fluorescence intensity (x-axis) of cells of gated NLCs stained with fluorochrome-conjugated isotype control mAbs of irrelevant specificity (open histograms) or a fluorochrome-conjugated mAb specific for a defined surface antigen (shaded histogram), as indicated in the upper right-hand corner of each panel.