Fucosylated selectin ligands, but not PSGL-1 alone, partially contribute to homing of DCs to inflamed skin.

(A) Immature DCs from Fuc-TVII–deficient mice (Fuc-TVII^−/−), Fuc-TIV/VII double-deficient mice (Fuc-TIV/VII^−/−), and wild-type control mice, as well as T cells from same strains were compared for their ability to home into the inflamed ear. Inhibition of homing in Fuc-TVII^−/−mice was statistically significant for T cells (P < .001), but not for DCs. Inhibition of homing in Fuc-TIV/VII^−/− mice was statistically significant for DCs (P = .048). (B) Immature DCs or T cells from Balb/c mice were incubated without antibody (no block) or with 50 μg 4RA10 per mouse (α-PSGL-1), or of the non–adhesion-blocking mAb 4RB12 against PSGL-1 (control mAb) prior to injection. Otherwise, the experimental protocol was identical to that described in Figure 10. Inhibition of homing due to blockade of PSGL-1 was statistically significant for T cells (P < .01), but not for DCs.