Fig. 9.
Rolling of immature DCs to P- and E-selectin in an adhesion assay under hydrodynamic flow.
(A) Immature DCs from Balb/c mice (left panel) and 32Dcl3 cells (right panel) were allowed to interact under flow with P-selectin–IgG (●) or E-selectin–IgG (▴) immobilized on glass coverslips that were inserted into a planar flow chamber with a variable flow rate of 1.0 to 3.2 dynes/cm2. (B) At a constant flow rate of 1 dyne/cm2, cells were either used directly (no block) or preincubated with 10 μg/mL of the blocking antibody 4RA10 against mouse PSGL-1 (α-PSGL-1) or 10 μg/mL of the mAb PS/2 against VLA-4 (control mAb). The depicted experiments represent 1 of 3 independent experiments with similar results.
