Binding of lineage-depleted and nondepleted immature DCs to P- and E-selectin under static conditions.
DCs were derived from bone marrow of Balb/c mice that had been depleted (Lin− culture) or not depleted (bulk culture) of Lin+ cells. Cells cultured for 5 days in the presence of GM-CSF and IL-4 gave rise to immature DCs. Cells were allowed to bind to immobilized P-selectin–IgG (A) or E-selectin–IgG (B) in 96-well microtiter plates (as indicated). Numbers on the y-axis refer to the number of bound cells/mm2 remaining in the wells after washing. For antibody-blocking studies, cells were preincubated either with 10 μg/mL 4RA10 (α-PSGL-1) or 10 μg/mL PS/2 against VLA-4 (control mAb). Human IgG refers to wells coated with plain human IgG1 as negative control.
