Fig. 7.
Binding of dendritic cells to P- and E-selectin in a static adhesion assay.
Immature and mature DCs from Balb/c mice were allowed to bind to immobilized P-selectin–IgG (A) or E-selectin–IgG (B) in 96-well microtiter plates. The 32Dcl3 cells served as a positive control. Numbers on the y-axis refer to the number of bound cells/mm2 remaining in the wells after washing. For antibody-blocking studies, cells were preincubated either with 10 μg/mL 4RA10 (α-PSGL-1) or 10 μg/mL PS/2 against VLA-4 (control mAb). Human IgG refers to wells coated with plain human IgG1 as negative control.
