CLA expression on the surface of DCs.

Immature and mature DCs from cultures of aliquots from the same bone marrow preparation were incubated with identical amounts of mAb HECA452 and subjected to flow cytometry. The cell lines 32Dcl3 and CHO DUKX B1 served as positive and negative controls, respectively. The open region represents the IgM isotype control and the filled region the HECA452 signal. Flow cytometry was performed as described in “Materials and methods.” The calculated peak maxima are indicated as numbers above the peaks.