Surface phenotype of in vitro–derived mouse DCs.

DCs were derived from bone marrow of Balb/c mice that had been depleted (Lin⁻ culture) or not depleted (bulk culture) of Lin⁺ cells. Cells cultured for 5 days in the presence of GM-CSF and IL-4 gave rise to immature DCs, whereas cells cultured for 9 days in identical medium and with LPS added for the last 24 hours gave rise to mature DCs. Flow cytometry was performed as described in “Materials and methods.” The mature and immature DCs depicted here originated from aliquots of the same bone marrow preparation.