Analysis of GFP expression in adenovirus-GFP–infected monocytes.

Monocytes were cultured overnight in SFM/G or infected with adenovirus-GFP at an MOI of 200. At 48 hours, cells were harvested, stained with either PE-labeled isotype-matched control antibodies or PE-labeled anti-CD83 mAb (x-axis), and subjected to multicolor FACS analysis to detect relative levels of expression of GFP (y-axis). In addition, phase contrast and fluorescent photomicrographs of both uninfected and infected cultures were taken to demonstrate GFP expression and virus-induced cell clustering.