Adenovirus-induced acquisition of DC phenotype in the absence of endotoxin contamination.

Adenovirus-induced acquisition of DC phenotype is not a result of endotoxin contamination. Monocytes were cultured in SFM/G for 24 hours and were then either infected with adenovirus-Luc (with and without polymyxin B), infected with heat-inactivated (HI) adenovirus-Luc, or treated with 10 ng/mL LPS (with or without polymyxin B). Monocytes were incubated with 200 ng/mL polymyxin B for 1 hour before the indicated treatments. All cells were harvested, stained, and subjected to FACS analysis 36 hours after infection or treatment. Results are representative of 6 separate experiments with different donors.