Distribution of ERM proteins on CEM-VBL100 cells.

Immunocytochemistry (APAAP, counterstaining with Mayer hemalum) (A) and immunofluorescence (B) analyses in CEM-VBL100 cells for ezrin (left panels), radixin (central panels), and moesin (right panels). Note the extreme level of polarization of all the proteins on pseudopods or uropods of CEM-VBL100 cells, independent of the detection method used. Bar = 5 μm.