Fig. 9.
The HDAC inhibitor TSA activates endogenous RAR transcriptional activity in EML but not EML/GM-CSF cells.
The indicated cells were transfected with the RXR/RAR-responsive RARE tk-LUC reporter and then cultured for 5 hours in the presence or absence of TSA (10−7 M). Relative luciferase activity was then determined on harvested cell extracts using a β-galactosidase reporter as an internal control. The results represent the mean of at least 3 independent experiments.
