SDS agarose electrophoresis VWF plasma multimer analysis before and after infusion of A-SD/HT (60 IU/kg VWF:RCof) in a representative patient with type 3 VWD.

Gels were prepared in 1.2% agarose. Lane 1, normal pooled plasma; lane 2, before infusion; lane 3, 15 minutes after infusion; lanes 4 through 9, 1, 3, 6, 12, 24, and 48 hours after infusion. Increased size in circulating VWF multimers was observed for more than 24 hours after infusion.