Splenic CD4⁺CD25⁺ T cells are decreased in μ-chain–deficient mice but not in NK T-cell–deficient mice.

Splenocytes from μ-chain–deficient mice, NK T-cell–deficient mice, and wild-type (WT) mice were stained with anti-CD25 PE and anti-CD4 FITC. Shown are representative FACS profiles of CD4 versus CD25 on the CD4⁺ population (A) and the absolute number of CD4⁺CD25⁺ T cells in spleen (B) from 4 to 5 mice in each group. *Significantly different from the mean value of wild-type mice; *P < .001.