Dual TCRα expression is not essential for the development of CD4⁺CD25⁺ T cells.

(A) Thymocytes from TCRα^+/− mice and TCRα^+/+ mice were stained with anti-CD3 FITC, anti-CD25 PE, anti-CD8 APC, and anti-CD4 PerCP. Shown are representative FACS profiles of CD4 versus CD25 on a CD4⁺CD8⁻CD3^high population. FACS profile of CD4 versus CD8 and cell number of thymocytes was indistinguishable between TCRα^+/− mice and TCRα^+/+ mice (data not shown). (B) Splenocytes from TCRα^+/− mice and TCRα^+/+ mice were stained with anti-CD25 PE and anti-CD4 FITC. Shown are representative FACS profiles of CD4 versus CD25 on the CD4⁺ population from 5 mice in each group.