CD4⁺CD25⁺ T cells preferentially express the second TCRα chain in DO10⁺ TCR transgenic mice.

Single-cell suspension of splenocytes from DO10⁺ mice (A) and DO10⁺ RAG-2^−/− mice (B) were stained with anti-TCR Vα2 FITC, anti-CD25 PE, anti-CD4 PerCP, and KJ1-26 biotin. After biotinylated antibody was visualized with streptavidin APC, cells were analyzed on FACScalibur. Shown are representative FACS profiles of CD4 versus CD25 of CD4⁺ splenocytes (upper panels) and KJ1-26 versus Vα2 (lower panels) on either CD4⁺CD25⁺ T cells or CD4⁺CD25⁻ T cells from 5 mice in each group.