![Fig. 2. Lymphocytes from evolutive B-CLL express a high level of P2X7R and are inhibited by ATP. / (A) RT-PCR amplification and detection were performed as described in “Study design”; 10 μL PCR product was loaded in each lane. Amplification products from 2 evolutive (E1 and E2) or indolent (I1 and I2) B-CLL patients are shown; human macrophages are shown as a control (Cont). In these same lymphocyte populations, increases in [Ca++]i ([Ca++]i) caused by stimulation with 1 mM ATP were also measured and reported below each lane. [Ca++]i increases are expressed as means ± SD of triplicate determinations. (B) β-actin amplification fragments. (C) Western blot with a specific anti-P2X7 serum (see “Study design”). (D) Lymphocytes, 106 per well, from 8 patients (4 B-CLL indolent and 4 B-CLL evolutive) were incubated as described in “Study design” for 24 hours in the presence or absence of 2.5 mM ATP. Data (cpm) are the means ± SD of quadruplicate determinations from a single experiment representative of 4 others. Statistical significance (P) between evolutive B-CLL incubated without or with ATP is reported above bars.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/2/10.1182_blood.v99.2.706/6/h80221988002.jpeg?Expires=1722774524&Signature=OFvJp~Pm77JTbhH5QKFfWxgyUTNzAkYYd9pf5z0JLxIZijFypeSG1aKuOQmg3Bwld4gyU~1WutgTX9lyT-op~4LmAkyTq7qKtkEIxnvsEtwFa6pNEPoEzF-wENSGlwDCrbwQESKLQGt9t-lkHr9P7~U5RpOJc5Z3m-~KgOjd23f5uSdlE-E3v6VdqiSikF-g8KqyoJW6-WtVPAw2pDXU-DqBFHfkadBQrWpM0fDuj8EeHCfYs89ZHCNi5JEC2NUi48kGMPV88VHMfyr~ejg6MtMlfW1YJWie2bLwV~WPv~OwRDnqxMJhbs1q1Cj8U~1RT1z8wp1tMsx-uTMnAvWVmg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Lymphocytes from evolutive B-CLL express a high level of P2X7R and are inhibited by ATP.
(A) RT-PCR amplification and detection were performed as described in “Study design”; 10 μL PCR product was loaded in each lane. Amplification products from 2 evolutive (E1 and E2) or indolent (I1 and I2) B-CLL patients are shown; human macrophages are shown as a control (Cont). In these same lymphocyte populations, increases in [Ca++]i ([Ca++]i) caused by stimulation with 1 mM ATP were also measured and reported below each lane. [Ca++]i increases are expressed as means ± SD of triplicate determinations. (B) β-actin amplification fragments. (C) Western blot with a specific anti-P2X7 serum (see “Study design”). (D) Lymphocytes, 106 per well, from 8 patients (4 B-CLL indolent and 4 B-CLL evolutive) were incubated as described in “Study design” for 24 hours in the presence or absence of 2.5 mM ATP. Data (cpm) are the means ± SD of quadruplicate determinations from a single experiment representative of 4 others. Statistical significance (P) between evolutive B-CLL incubated without or with ATP is reported above bars.
