CMo-dependent tyrosine phosphorylation of STAT1 and STAT5.

Immunoblot analysis of WCEs (15 μg) of TF-1 cells either grown in IL-3 for 1 and 4 days (lanes 1-2) or starved of IL-3 for 1 day (lane 3) or incubated in IL-3–deprived medium with CMo or BMo HTLV-2 strains for 1 and 4 days (lanes 4-7), respectively. In the first panel from the top, the filter was hybridized with anti–phospho-STAT5. In the second panel the filter, after stripping, was hybridized with a mixture of anti-STAT5A and anti-STAT5B antibodies. In the third panel, a new filter containing the same samples shown in the other panels was probed with anti–phospho-STAT1 and, after stripping, with anti-STAT1 antibody (last panel).