CMo, but not the BMo HTLV-2 strain, induces STAT1 and STAT5 activation in TF-1 cells.

(A) EMSA using the PRE probe and 8 μg WCE from TF-1 cells either grown in IL-3 (lanes 1-2) or starved from IL-3 (lanes 3-4) or incubated in IL-3–deprived medium with CMo or BMo HTLV-2 strains for 1 and 4 days (lanes 5-8), respectively. STAT/DNA-binding activity was positive (lanes 1-2) and negative (lanes 3-4) in TF-1 cells grown in the presence or in the absence of IL-3, respectively. (B) Supershift analysis using the PRE probe; 8 μg WCE from TF-1 cells grown in IL-3 (lanes 1-2) or incubated with CMo virus in IL-3–deprived medium for 1 day and anti-STAT1 (1), anti-STAT5 antibodies raised against a C-terminal (5C) or an N-terminal (5N) epitope (lanes 3-6). (C) Cord blood–derived CD34⁺ cells were incubated with CMo for 24 hours and STAT1 (1), and STAT5 (5) activation was determined by supershift analysis using the PRE probe on WCE (8 μg). Starv indicates starved from IL-3.