Fig. 7.
![Fig. 7. Antigen-presenting cells in allogeneic full-term fetal blood recipients. / Spleen cells were obtained between 148 and 258 days after transplantation for the following assays. (A) Absolute number of splenic dendritic cells. Spleen cells were stained with anti-CD11c, CD3, CD4, B220 and Gr-1 antibodies simutaneously. Splenic dendritic cells were defined as CD11c+CD3−CD4−B220−Gr-1−. (B) Functional analysis of antigen-presenting cells by MLR. Spleen cells were irradiated (20 Gy) and used as accessory cells to stimulate spleen cells from BALB/c mice (2.5 × 105/well). Proliferation was determined by incorporation of [3H]thymidine. Each group contained 5 animals.*P < .01, compared with other groups.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.364/6/h80121925007.jpeg?Expires=1724247646&Signature=Mu19u22Gwx~7RO07F34yQI3jMUmyCjendBGKNznPrWpAu4EEhkXSKbQKqB~G044yoEEiCPbpGXhIoZnD2Cc0KxDvUw7vyHio0VLKOkGfHPtvz8s6KFcUtkUkL7tTiZZ~8iroZmg0iM4YdOVVQbjOhrermiN85FPoRfMHIVFXgLzP9fvPfHYVdv6sQ8wRdqDY6kkcoHTrE73baSLF6HOtPh5QX3qdDo9rHRA~Z7GJ-J0YHxro5VgXwMaOiEbawZpWeGtgOA3ozUG3YJo-0MXm5LJkDyGDLxAdBx6d8DEV6CWdPp88~txZN-Y-ZyUDplIkkV9sSc7m2sJo4giaOy--BA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Antigen-presenting cells in allogeneic full-term fetal blood recipients.
Spleen cells were obtained between 148 and 258 days after transplantation for the following assays. (A) Absolute number of splenic dendritic cells. Spleen cells were stained with anti-CD11c, CD3, CD4, B220 and Gr-1 antibodies simutaneously. Splenic dendritic cells were defined as CD11c+CD3−CD4−B220−Gr-1−. (B) Functional analysis of antigen-presenting cells by MLR. Spleen cells were irradiated (20 Gy) and used as accessory cells to stimulate spleen cells from BALB/c mice (2.5 × 105/well). Proliferation was determined by incorporation of [3H]thymidine. Each group contained 5 animals.*P < .01, compared with other groups.
