![Fig. 6. Decreased immune responses against alloantigens in vitro of allogeneic full-term fetal blood recipients. / Mice were challenged with third-party newborn C3H/HeJ hearts in vivo 45 to 82 days before the assay. Spleen cells were isolated 200 days after transplantation and stimulated in vitro with irradiated C3H/HeJ spleen cells for proliferation and CTL assay. Sera were obtained on the same day for alloantigen-specific cytotoxic antibody assay. (A) Proliferation was determined by [3H]thymidine incorporation. (B) Cytotoxicity was assessed with51Cr-labeled 2-day Con A–activated C3H/HeJ spleen cells. Nonspecific release against irrelevant antigens (B10.D2) was performed to ensure the specificity of killing. (C) Third-party alloantigen-specific cytotoxic antibody was detected by microcytotoxicity assay. Two-day Con A–activated C3H/HeJ spleen cells were used as target cells. Dead cells were visualized by trypan blue. Each group contained 2 to 5 animals. *P < .05, compared with allogeneic T-cell–depleted bone marrow recipients.#P < .01, compared with normal C57BL/6 mice. TCD indicates T-cell–depleted.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.364/6/h80121925006.jpeg?Expires=1724246200&Signature=r7BkA6SHlBa~n0N-g1qTKPsJeadHMD39hfZUrMUMliPlm7Y4IU35X6DIzp5kSiA4hpuU9LkL4HPjfmAjF1Upo17hYu3mt~-ZTcXhecPzBsyxXKFT~4yPcgJ0BZrZMe0mtV9BPFCgaQKDWW4Nns~xJepfA64ZtoQAkrD16EcRTnLJJLjpQmvW46Gdd0N59jSLvgd~LZ8FbTAfR9tvwfdhNegtgZsY9Nej3HY8heb~FkFcDg56w6fZ84UgLMVkX~mH74MjkQ7Y40Z~EdUDGt8Sx13nLdefZLEqFBx5XxA~3PQca9Zojo6MhFYrgqQQ-06rwxRJwkMIPqqWdMxShAVZbg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Decreased immune responses against alloantigens in vitro of allogeneic full-term fetal blood recipients.
Mice were challenged with third-party newborn C3H/HeJ hearts in vivo 45 to 82 days before the assay. Spleen cells were isolated 200 days after transplantation and stimulated in vitro with irradiated C3H/HeJ spleen cells for proliferation and CTL assay. Sera were obtained on the same day for alloantigen-specific cytotoxic antibody assay. (A) Proliferation was determined by [3H]thymidine incorporation. (B) Cytotoxicity was assessed with51Cr-labeled 2-day Con A–activated C3H/HeJ spleen cells. Nonspecific release against irrelevant antigens (B10.D2) was performed to ensure the specificity of killing. (C) Third-party alloantigen-specific cytotoxic antibody was detected by microcytotoxicity assay. Two-day Con A–activated C3H/HeJ spleen cells were used as target cells. Dead cells were visualized by trypan blue. Each group contained 2 to 5 animals. *P < .05, compared with allogeneic T-cell–depleted bone marrow recipients.#P < .01, compared with normal C57BL/6 mice. TCD indicates T-cell–depleted.
