Long-term engraftment potential of murine full-term fetal blood in MHC-mismatched adult recipients.

Peripheral blood was obtained from allogeneic full-term fetal blood recipients at least 100 days after transplantation. The whole blood samples were stained with FITC-conjugated anti-H-2D^b(donor) and TC-conjugated anti-CD45 monoclonal antibodies simultaneously (see “Materials and methods”). The histograms were gated on CD45⁺ cells. The normal percent levels of H-2D^b+ cells for C57BL/6 (positive) and BALB/c (negative) mice are 99.8% ± 0.1% and 0.09% ± 0.06%, respectively. (A) Representative histograms. The light gray lines represent recipient-derived cells. (B) Long-term engraftment potential of full-term fetal blood and T-cell–depleted bone marrow. Each group contained 3 to 9 animals. P < .05, allogeneic full-term fetal blood groups versus allogeneic T-cell–depleted bone marrow groups; not significant, allogeneic full-term fetal blood groups versus normal C57BL/6; P < .001, allogeneic full-term fetal blood groups versus normal BALB/c.