Fig. 3.
![Fig. 3. Platelet-dense granule secretion is inhibited by PECAM-1 signaling. / Platelets were loaded with [3H]5-HT before stimulation with Cvx (62.5 ng/mL) or thrombin (0.1 U/mL). Where required, PECAM-1 was cross-linked an agonist was added (as described in “Materials and methods”). Secretion of [3H]5-HT into cell medium was measured using scintillation spectrometry. [3H]5-HT release is expressed as a percentage of total tissue content after subtraction of basal secretion values. Results represent mean ± SE (n = 3). Student t test was used to compare PECAM-1 cross-linked and non–cross-linked sample for statistical significance. *P < .05. PECAM-1 XL, PECAM-1 cross-linking](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.137/6/h80121926003.jpeg?Expires=1722856686&Signature=bcp-Y~t6N0GEoHcJtvUloPevFgkYZUuomedm62AsCiNtVoBKW0H71AYd1M36Y88pdMyeSed3NrDSjIp8kTYYegbzCCT1-92eJ-WDre9X~vzMKUg1FWcGtVvTBYb~1vWaO0fUnmuO4xzWv~tHbfrN8GdPL99amGFM74bXJh4tOVi3jJ5XtoG3T4dzVIKXHEeTWP6~NW3H8eD62-rb4QRnPhu3W15zrqdQ12tjnD6q--yRRsyiEvNxkhcWDmyPGoezcOd6MZcg4gd11ae4iNW5lEw1min1v5g8MqowKQAG1Ei6C3dEdvihKinM96IzC8FS7Ou9CiiNgdVhHO8vLXQo9Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Platelet-dense granule secretion is inhibited by PECAM-1 signaling.
Platelets were loaded with [3H]5-HT before stimulation with Cvx (62.5 ng/mL) or thrombin (0.1 U/mL). Where required, PECAM-1 was cross-linked an agonist was added (as described in “Materials and methods”). Secretion of [3H]5-HT into cell medium was measured using scintillation spectrometry. [3H]5-HT release is expressed as a percentage of total tissue content after subtraction of basal secretion values. Results represent mean ± SE (n = 3). Student t test was used to compare PECAM-1 cross-linked and non–cross-linked sample for statistical significance. *P < .05. PECAM-1 XL, PECAM-1 cross-linking
