Correlation of cytokine-induced expression of IRF-1 and CD40.

Time-dependent effects of IFN-γ (1000 U/mL) or of TNF-α (100 U/mL) plus IFN-γ (1000 U/mL) on IRF-1 and CD40 mRNA expression in the cultured HUVECs, as judged by RT-PCR analysis (calculated as percentage of basal gene expression). Qualitatively identical results were obtained with at least 2 further batches of cells.