Sequence analysis of tetramer-selected populations.

The panel on the left shows the postculture TCRBV spectratypic profiles for BV6.1 of donor 01, including the unselected, the HLA-B*0702 tetramer–selected, and HLA-A*0201 tetramer–selected populations. Seven and 10 clones representing the major peaks (shaded) of the HLA-B*0702 tetramer–selected and HLA-A*0201 tetramer–selected populations, respectively, showed identical sequence, indicating that each size class peak is composed of a single or very limited number of clones. The sequence data confirm a lack of conservation of CDR3 motifs between the clones selected by the different tetrameric complexes.