Phenotypic analyses of preculture and postculture cells from donor 01.

Cells were dual-stained with PE Cy5–conjugated anti-CD8 mAb and PE-conjugated HCMV pp65–specific HLA-B*0702 tetrameric complexes. The single parameter histograms are gated onto the CD8⁺ cell population and demonstrate an increase in the population of cells binding the tetrameric complexes over the period of culture.